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A selective medium was developed for isolation of Cephalosporium gregatum from soil. This medium (pH 5.5) consisted of the following ingredients per liter of distilled water: galactose, 5.0 g; peptone, 5.0 g; KH2PO4, 1.0 g; MgSO4⋅7H2O, 0.5 g; sodium borate, 0.5 g; PCNB, 0.5 g; sodium cholate, 0.5 g; streptomycin sulfate, 0.2 g; tetracycline HCl, 0.05 g; agar, 20g. This medium was more efficient for the quantitative detection of propagules of C. gregatum from soil than ASA, GWA or Martin medium. With this medium, populations of C. gregatum in many infested adzuki bean field soils were estimated and it was found that there was high correlation between disease index and populations of C. gregatum. Furthermore, by sieving technique it was confirmed that C. gregatum propagules could survive in soil.

A selective medium for isolation of Cephalosporium gregatum from soil and populations in adzuki bean field soils estimated with the medium.