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Circulating tumour cells (CTCs) are malignant tumour cells which detached from the primary tumour and circulate in peripheral blood (Thiery, Acloque, Huang, & Nieto, 2009). Recent discoveries show that classical epithelial CTCs can undergo epithelial-mesenchymal transition, which changes their phenotype to mesenchymal (Cichon, Nelson, & Radisky, 2015; Thiery et al., 2009). Mesenchymal CTCs can acquire additional malignant properties. Due to their rarity and heterogeneity CTCs can be best characterized on the level of single cell (Micalizzi, Haber, & Maheswaran, 2017; Palmirotta et al., 2017).

Development of a protocol for isolation and gene expression analysis in single circulating tumour cells