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Quantitative measurements of the properties of a natural multicomponent system, such as Municipal Wastewater (MWW) and Activated Sludge (AS), bring challenges in terms of analytical chemistry. In the present paper, we use the ‘Binary Model’ (BM), which is considering two kinds of particles of different nature able to absorb each other to form only one type of complex. This extremely simple modeling allows explaining non-intuitive measured optical properties behavior of MWW-AS mixtures. The experimental methodology used is very simple and relies on the measurements of the optical density of the mixtures by spectrophotometry. We derive a simple but realistic expression giving the optical density resulting from light scattering of mixed suspended particles. We report a series of experimental data obtained when characterizing samples of MWW and AS and illustrate the efficiency of the BM. This simple calculation already makes it possible to optimize strategies of flocculation/ coagulation of solids. We thus develop the bases of a simple and inexpensive methodology, able to provide information on complex particulate interactions which elucidation would otherwise require the use of very sophisticated analytical techniques.

Experimental characterization of the interactions between bacterial floc and suspended solids by means of optical density measurements.