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Aim: The purpose of this article is to prove the possibility to transfer a good amount of cells of the SVF(and ADSCs) in the infranatant portion of microfragmented adipose tissue.Method: The adipose tissue harvesting procedure is performed under local anaesthesia. The adipose tissuewas harvested with a 2 mm diameter microperforated cannula with 1 mm side port holes, mounted insidethe special patented guide. Both cannula and guide are included in the SEFFIHAIR™ medical device. Oncethe adipose tissue is harvested, it is gently washed, and it was divided in two specimens: (EMU) the tissuewas emulsified with 20 passages from one syringe to another and (CTRL) the tissue didn’t undergo anyemulsification.Results: The emulsification procedure liberated alive and proliferating cells and we observed that thespecimens derived with a 1 mm side port hole cannula and then emulsified (EMU) showed a higher numberof cells in the infranatant part compared to the one derived from the control tissue without any (1 EMU vs.1EMU infra).Conclusion: The use of microcannulas, in combination with a mechanical digestion by an emulsificationprocedure and centrifuge, could ease SVF cells isolation for regenerative treatment and could also beperformed in a medical facility.

Infranatant Portion of Microfragmented Adipose Tissue: A Promising Source of SVF for the Management of Androgenetic Alopecia