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Bacterial resistance to antibiotics is an important public health problem that affects human, veterinary, food and environmental security in Perú. The objectives of this work have been the phenotypic and genotypic detection of Escherichia coli strains that produce extended spectrum β-lactamase enzymes (βLEE), isolated in poultry in a private laboratory located in the city of Lima, Peru. A total of 185 isolates of E. coli from birds of 26 intensive breeding companies located in Peru were evaluated, to which necropsies were performed during the period from November 2015 to November 2016. E. coli producing extended-spectrum β-lactamase enzymes (βLEE) were detected according to the double disc synergy confirmatory method according to the Antibiogram Committee of the French Society of Microbiology (Jarlier Method), while genotypic characterization was performed by the amplification of blaCTX-M-1, blaTEM and blaSHV genes in PCR. The results reflect a total of 185 isolations, found 38.4% of strains with the presence of ESBL confirmed phenotypically; Likewise, genes were expressed from total DNA by polymerase chain reaction (PCR) obtaining 33.5% between the blaTEM, blaSHV and blaCTX-M-1 genes and 4.9% of E. coli strains producing βLEE phenotypic, no gene bla determined. In conclusion, the presence of Escherichia coli strains that produce extended-spectrum βlactamase enzymes (βLEE) is high, with the blaCTX-M-1 gene being the most frequent, therefore, it is necessary to increase detection and control measures. control of origin of this microorganism producing these enzymes, in the same way to guide the empirical antimicrobial

DETECCIÓN FENOTÍPICA Y GENOTÍPICA DE ESCHERICHIA COLI PRODUCTORAS DE Β-LACTAMASAS ESPECTRO EXTENDIDO AISLADAS DE AVES DE ABASTO EN PERÚ