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vi CHAPTER 1: INTRODUCTION 1 General introduction 1 Dissertation organization 3 Literature review 5 References 15 CHAPTER 2: ENGINEERING WITH PRECISION: TOOLS FOR THE NEW GENERATION OF TRANSGENIC CROPS 22 Abstract 22 Introduction 22 Concluding remarks 38 Acknowledgements 39 References cited 39 CHAPTER 3: A BACTERIAL SIGNAL PEPTIDE IS FUNCTIONAL IN PLANTS AND DIRECTS PROTEINS TO THE SECRETORY PATHWAY 46 Abstract 46 Introduction 47 Materials and methods 50 Results and discussion 56 Acknowledgements 67 References 67 Figure legends 71 CHAPTER 4: WET MILLING TRANSGENIC MAIZE SEED FOR FRACTION ENRICHMENT OF RECOMBINANT SUBUNIT VACCINE 81 Abstract 8

Maize for production of recombinant proteins: fundamental and practical considerations