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This!thesis!represents!efforts!made!in!technological!developments!for!the!study!of! metabolic!biology!in!plants,!specifically!maize,!using!matrixYassisted!laser! desorption/ionizationYmass!spectrometry!imaging!(MALDIYMSI).!The!first!chapter!gives!a! brief!introduction!on!what!MALDIYMSI!is!and!how!it!works.!The!final!chapter!provides!a! summary!of!all!works!in!this!thesis!as!well!as!providing!future!directions!that!may!be! pursued!based!on!this!research.! ! The!second!chapter!encompasses!research!performed!tracking!metabolite! distributions!between!two!different!inbreds!of!maize!seeds!throughout!the!course!of! germination!utilizing!GCYMS,!LCYFluorescence,!and!MSI!analysis.!GC!and!LC!data!provide! quantitative!information!regarding!a!wide!range!of!metabolites!at!crude!localization!levels,! while!MSI!is!able!to!track!localizations!of!limited!metabolites!in!fine!spatial!detail.!Results! demonstrate!that!metabolites!are!differentially!localized!throughout!the!seed!depending!on! inbred,!metabolite!class,!and!germination!time!point.! ! The!third!chapter!demonstrates!how!MALDIYMSI!can!be!used!to!acquire!large,! metabolomic!scale!datasets.!Serial!sections!of!a!maize!seed!are!coated!with!various! matrices!and!analyzed!in!positive!and!negative!ion!mode!using!a!multiplex!imaging! strategy.!This!strategy!allows!for!visualization!of!metabolites!through!MSI!as!well!as! metabolite!identification!through!the!collection!and!analysis!of!highYquality!MS/MS! spectra.!! ! The!fourth!chapter!outlines!the!development!of!a!binary!matrix!comprised!of!2,5Y dihydroxybenzoic!acid!and!iron!oxide!(Fe3O4)!nanoparticles.!The!matrix!is!shown!to! alleviate!the!suppression!of!triacylglycerol!species!by!phosphatidylcholine,!both!through!

Technological development of high-performance MALDI mass spectrometry imaging for the study of metabolic biology