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By definition, a macroinvertebrate is any invertebrate that is large enough to be retained on a 500-μm mesh sieve. Although macroinvertebrates (hereafter invertebrates) are important in food webs and sensitive to environmental conditions, they are often omitted from wetland condition monitoring programs because use of this fine-mesh sieve makes sample collection and processing time consuming and expensive. The objective of this study was to identify a more cost-effective approach for obtaining invertebrate-based data that can be used to evaluate wetland condition. In 2014 and 2015, invertebrates and associated particulate matter (PM; living and dead plants, sediment) were collected from 27 wetlands in the Iowa Prairie Pothole Region using a stovepipe sampler. Sample material retained on a 500-μm mesh sieve was preserved. In the laboratory, samples were washed through a series of sieves that separated invertebrates and particulate matter into four size fractions. The 6-mm fraction included material retained on the 6-mm sieve, the 4-mm fraction included material retained on both 4and 6-mm sieves, the 2-mm size fraction included material retained on 2-, 4-, and 6-mm sieves, and the 500-μm fraction was the sum total of material retained on 2-, 4-, 6-, and 500-μm sieves (i.e., the entire sample). Volume of each sample size fraction and time required to separate invertebrates from PM were recorded. Invertebrates were identified to family (mollusks, insects, isopods), order (amphipods, decapods), or class (annelids), and numbers of individuals were recorded. Subsequently, invertebrates were again washed through the sieve column in the absence of PM, and organisms in each size fraction were identified and counted. Values for invertebrate numerical density and taxon richness variables were quantified for each of the four invertebrate size fractions and when PM was present (+ PM) and absent (PM). Invertebrate assemblage variables were related to environmental variables indicative of wetland condition (e.g., turbidity, chloride concentration,

Evaluating alternative macroinvertebrate sampling methodologies in wetlands: Influence of sieve mesh size on relationships between environmental and assemblage variables