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Article Info Milk is an excellent medium for the growth of many bacteria. The aim of this study was to determine antibiotic profiling and thermal inactivation of Staphylococcus aureus and Escherichia coli isolated from raw milk of Dharan. Total viable, total staphylococcal, and total coliform counts were carried out by conventional microbiological methods. Identification was done based on Gram’s staining and biochemical tests. The antibiotic susceptibility test was carried out by the modified Kirby-Baur disc diffusion method. Thermal inactivation of the isolates was carried out by subjecting them to thermal treatment in a water bath. Total plate count ranged from 204×10 CFU/mL to 332× 10 CFU/mL. Total staphylococcal and total coliform counts ranged from 14×10 CFU/mL to 8×10 CFU/mL and 11×10 CFU/mL to 3×10 CFU/mL respectively. S. aureus showed an increasing resistance patterns towards Ampicillin, Cefotixin, Carbenicillin and Cefotaxime. Ciprofloxacin, Erythromycin, Amikacin, Gentamycin, Azithromycin, and Chloramphenicol were found to be effective against S. aureus. All the E. coli isolates were resistant to Ampicillin and least resistant to Cefotixin. Chloramphenicol, Amikacin, Azithromycin, and Nalidixic acid were found highly effective in E. coli. The D-values for S. aureus at 56° C, 58° C and 60° C were 1.36 min, 1.19 min, and 1.09 min respectively. The Z-value was 14.92° C. While D-values were obtained as 0.98 min, 0.75 min, and 0.57 min for E. coli at 56° C, 58° C and 60° C respectively, and Z-value was 9.75° C. Hence, S. aureus was found to be more heat resistant than E. coli. Article history: Received date: 24 May 2020 Accepted date: 20 October 2020

Antibiogram Profiling and Thermal Inactivation of Staphylococcus aureus and Escherichia coli Isolated from Milk of Dharan, Nepal