Open AccessA Simplified Method for Determination of Pyridoxal Phosphate in Biological Samples
Author(s) -
Arne Hamfelt
Publication year - 1986
Publication title -
upsala journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.808
H-Index - 41
eISSN - 2000-1967
pISSN - 0300-9734
DOI - 10.3109/03009738609178496
Subject(s) - decarboxylation , medicine , chromatography , tyramine , tyrosine , high performance liquid chromatography , pyridoxal 5 phosphate , pyridoxal , phosphate , biochemistry , chemistry , catalysis
A new method for the determination of pyridoxal phosphate in plasma and in cerebrospinal fluid is described. It is based on the generally accepted apotyrosine decarboxylase method, but, in stead of following the decarboxylation rate using 14C- och 3H-labelled tyrosine, a high performance liquid chromatography (HPLC) method for separation of tyrosine and tyramine is used. This procedure is recommended as it is less time consuming and avoids utilizing radioactive material.