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The study applies experimental approaches to spore germination to determine whether the germinants will affect the two different species B. cereus and B. anthracis using in vitro tests. Carrying out studies on the vaccine strain of the B. anthracis, we used different combinations of amino acids to study possible ways to improve spore germination. The best results were obtained using inosine and L-serine as well as inosine with L-alanine and L-valine (0.1 mM aqueous solution of an equal ratio of amino acids was used). Analysis of the results of the studies showed that part of spores germinated in nutrient media after treatment with a mixture of amino acids reached 86.2% compared with 58% in the control. The variability of these values for tested strains ranged from ± 3.8% to ± 13.6%. We also found that the percentage of germinated spores does not increase significantly in time after the first 48 hours from the start of cultivation

. Influence of aminoacids on Bacillus genus bacteria spore germination