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The Lactobacillus is rich in whey it can be easily isolated from the whey sample. In the present study, lactobacillus sp was isolated from whey sample collected from Aavin Dairy industry, Madurai. The raw milk sample was enriched in peptone water. It was then plated on MRS medium by spread plated method. The selection of acid producing organism was done in MRS medium supplemented with calcium carbonate and L-cystein. The selected isolate was identified based on morphological and biochemical characterization tests and it was confirmed that the isolate belonged to the genus lactobacillus in accordance with the bergey’s manual of determinative bacteriology. Before evaluating the isolated lactobacillus as probiotics against selected pathogens, Lactobacillus was tested for its tolerance against sodium chloride, acidic pH, Ox bile tolerance etc. Bacteriocin was extracted using MRS medium and it was then partially purified using salt aggregation method. The partially purified bacteriocin was the mixed with SDS containing phosphate buffer for testing the antimicrobial activity. The partially purified bacteriocin protein was tested against clinical pathogens like E.coli, P.aeruginosa, P.fluoresence and Klebsiella pneumonia using agar well diffusion method. Bacteriocin is proteinaceous in nature. The molecular weight of partially purified bacteriocin was detected by SDS-PAGE. The quantitative estimation of organic acid was determined for the isolated Lactobacilli. Antioxidant property of the lactobacillus was detected by reducing power assay method.

Probiotic Efficiency of Lactic Acid Bacteria from Milk Treated against Gastro Intestinal Pathogens