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The X. americana leaf was extracted using water by cold extraction method. AgNPs were synthesized by phytoreducing method. The synthesized silver nanoparticles were characterized by using different techniques including UV–visible spectroscopy, FTIR analysis, scanning electron microscopy and energy dispersive X-ray spectroscopy. In-vitro antioxidant activity of synthesized AgNPs were evaluated by using FRAP, H2O2, and DPPH assays, anti-inflammatory activity was carried out by using protein denaturation in-vitro bioassay and anthelmintic activity by employing standard in-vitro method (Pheretima posthuma model). UV-Vis spectral analysis which revealed a higher absorbance peak (λmax) at 413 nm. FTIR spectra showed shifts in some peaks of aqueous extract with functional groups. SEM studies show the size of AgNPs in the range of 30-150 nm with irregular shape. EDX shows sharp peak of silver at 3 kev with various elemental composition. In-vitro pharmacological study revealed that synthesized AgNPs exhibited potential antioxidant, anti-inflammatory and anthelmintic activity.

Pharmacological Applications of Silver Nanoparticles Synthesized using Aqueous Leaf Extract of Ximenia americana