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Coxiella burnetii (C. burnetii) is negative Gram bacteria that caused zoonotic disease Q fever. The main reservoir of this disease are ruminants such as cow, goat, and sheep. Q fever infection happened to human relatively easily through aerosol, direct contact, and consumption of animal products from infected animal that has not been appropriately handled. This disease spread to almost all corner of the world and is a threat to food security and bioterrorism. Identification of C. burnetii infection in beef cattle in Indonesia is still limited. This research aim was to detect C. burnetii infection in various organs of beef cattle from Depok, Indonesia, through molecular examination using nested PCR (nPCR). The result of this research showed that the samples are identified to be positive of Q fever through nPCR method in various organs. Distribution of infection detected on heart, spleen, and kidney that mostly found in spleen and heart. This result indicates that C. burnetii probably can caused endocarditis in beef cattle like in human. This result also give a warning to more attention to the zoonotic threats for human in slaughter house. Keywords—Q fever, Depok, nested PCR

Detection of Coxiella burnetii Infection in Various Organs from Beef Cattle in Depok, West Java, Indonesia