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-Humans use water to support life. The objective of the study was to know the quality of hygiene and sanitation, physical quality (Odor, Color, TDS, Flavor, Turbidity, Temperature), chemical (Arsenic, Flouride, Total Chromium, Cadnium, Nitrate, Nitrite, Cyanide, Aluminum, Fe, Chloride, Manganese, Ammonium), and microbiology (Total Bacteria Coliform and E.Coli) on Drinking Water Refill (DAMIU) in work area of Puskesmas Lingkar Barat Bengkulu City on 2017. This research type was descriptive, qualitative and quantitative. Population and sample in this research were entrepreneur of Depot of Drinking Water Refill in work area of Puskesmas Lingkar Barat Bengkulu City as much as 12 DAMIU. Technique data analysis of drinking water quality data was conducted univariat by describing the characteristics of each research variable based on the standard method of Regulation of the Minister of Health of the Republic of Indonesia No. 43 of 2014 on Sanitation Hygiene of Drinking Water Refill Depot (DAMIU) and Number 492/2010 on Water Quality Requirements. More than 7 (58.67%) of DAMIU meet sanitary hygiene requirements, the results of inspection of refill drinking water available at DAMIU physically and chemically 100% meet drinking water health requirements, and more than some (66.67%) of DAMIU in the work area of the Puskesmas Lingkar Barat Bengkulu City were microbiologically eligible for consumption. Suggestions can be taken into consideration for local government and health center to monitor the quality of refill drinking water more and more circulating in the community to be guaranteed quality of quality and safety.

Physical Examinations, Chemicals and Microbiology on Drinking Water Refill