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Hydrilla verticillata is one of the aquatic plants that contain some secondary metabolites, such as triterphenoids and steroids. The aim of this study was to determine the toxicity levels of steroids isolated from ethyl acetate fraction of Hydrilla verticillata separated by column chromatoghraphy. The biomass of Hydrilla verticillata was dried and powdered. Hydrilla verticillata powder is then extracted by maceration methods using ethanol solvent. The crude ethanol extracts were hydrolyzed using 2 N of HCl and partitioned using ethyl acetate solvent. The steroids compounds of ethyl acetate fraction were separated by Column Chromatography. The steroid isolates from Column Chromatography were identified by FTIR and UV-Vis spectrophotometer. The toxicity level of Column Chromatography steroid isolates was determined by BSLT method. The result of this research showed that extraction through maceration using ethanol produced 2.52 % yield, whereas the percent yield of the partition using ethyl acetate was 45.04 %. Separation by column chromatography resulted 3 steroids isolates B1, B2, and G1. The toxicity test showed that column chromatography steroids isolate from ethyl acetate fraction of Hydrilla verticillata has toxicity properties. The LC50 value of B1, B2 and G1 isolate was 14.58, 7.55 and 4.71 ppm, respectively.

Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata