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The peel of citrus fruits contains a large percentage of pectin which can be a good substrate for pectinolytic microorganisms. These microbes secrete large amount of extracellular enzymes to degrade the cell wall of substrates. The current study was conducted for the production and characterization of pectinase from fungal strains using citrus fruits peel as a substrate. The optimum pectinase production was analyzed from pectinase activity assay. A. niger,A. flavus and A.fumigatus, showed maximum production at 6th day,and A. oryzae,at 4 th day. The optimum temperature for pectinase production by A. niger and A. oryzae was found to be 35Â°C and for A. flavus and A. fumigates was found to be 40Â°C.Pectinase showed optimum pH in the range of pH 4.5-5.0. Pectinase was purified by the addition of 60% of ammonium sulfate precipitation and dialysis and showed various fold increase in pectinase activity.

PRODUCTION AND PARTIAL PURIFICATION OF PECTINASE BY FUNGAL STRAINS GROWN ON ORANGE PEEL