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The nanocomposite films of polypyrrole (PPy) and functionalized multiwalled carbon nanotubes (MWCNT) were electrochemically synthesized on stainless steel in 0.1 M aqueous solution of dodecylbenzene sulfonic acid. The films deposited on electrode were used for the immobilization of enzyme for determination of lactate in sweat. The enzyme, lactate oxidase (LOD) was immobilized by cross-linking via glutaraldehyde onto PPy/MWCNT film deposited on stainless steel. The immobilization was done by cross-linking 2 mg/ml LOD via 0.1% glutaraldehyde at 0.1 M phosphate buffer with 7.0 pH. The synthesized composite films were characterized using Cyclic Voltammetry (CV), Fourier Transform Infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). The porous morphology of the composite films and ability to promote electron transfer reaction of MWCNT fillers leads to high enzyme loading and an increase in lifetime, stability and fast response time of the enzyme electrode. These PPy/MWCNT/LOD electrodes when tested for lactate in artificial sweat have shown a detection limit of 0.1 mM/l, a response time of about 60s and a shelflife of about 2 weeks with a linear response range from 0.5 to 6 mM.

FABRICATION OF POLYPYRROLE / MWCNT NANOCOMPOSITE FILMS AS LACTATE BIOSENSOR FOR SWEAT ANALYSIS