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Culex tritaeniorhynchus  and  Culex vishnui    are medically important mosquitoes that transmit Japanese Encephalitis (JE) virus. There is less information about the recording data and research due to genetic character differences among them. The objective of this study was to examine the genetic variation of  Cx. tritaeniorhynchus  and  Cx. vishnui  in 3 site of Central Java using PCR-RAPD.  The study was done from January to November 2017, in Pekalongan City, Pekalongan Regency and Semarang Regency. Adult female mosquitoes were collected by landing bite method. DNA of ten  Cx. tritaeniorhynchus  samples and fifteen samples of  Cx. vishnui  were purified using  DNA kit extraction. Furthermore, PCR amplification was conducted with 5 RAPD primers (OPA 11, 12, 15, 16, and 20) and would run into 2% gel electrophoresis for 45 minutes. Cluster analysis was done by using MVSP TM  software. The results showed  213 genetical characters of  Cx. vishnui,  while 142 characters were shown by  Cx. tritaeniorhynchus . The dendograms showed 3 distinct grups of  Cx. vishnui  from 2 sites of Pekalongan and 1 site of Semarang, while  Cx. tritaeniorhynchus  showed 2 distinct grups, which were 1 group from Pekalongan and 1 group  from Semarang. Low genetic similarity (u003c10%) was shown  Cx  . vishnui  from Pekalongan City and Pekalongan District, and there was no genetic similarity in  Cx  . tritaeniorhynchus  from Pekalongan and from Semarang. It is concluded that the polymorphism of  C  x tritaeniorhynchus  and  Cx  . vishnui  reached 100%.

Phylogenetic Analysis of Culex tritaeniorhynchus and Culex vishnui Vector of Japanese Encephalitis Virus