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Received: Revised: Accepted: Published online: February 19, 2019 June 20, 2019 July 03, 2019 August 15, 2019 It is observed that different Nramp1 genotypes are linked with Brucella resistance or susceptibility. So, current study aimed to find the Brucella resistance and susceptibility of local and exotic breeds of Pakistan on the basis of Nramp1 genotypes. Two breeds of buffaloes and five breeds of cattle were screened for brucellosis. The overall seropositivity determined by RBPT in cattle and buffaloes was 77.5% and 33.5% respectively. PCR results showed 54.5 and 25% brucella positive cases in cattle and buffaloes respectively. Nili-Ravi buffaloes pertain resistant genotype Nramp1 BB as compared to Kundi breeds of buffaloes possessed susceptible genotype Nramp1 AA. Similarly, Sahiwal breed was more resistant for brucellosis compared to cross breeds and Friesian. Phylogenetic analysis showed that Nramp1 gene of local breed had more closeness with B. taurus of China. This gene resistance can be used for genomic selection of animals to reduce disease cost and economic losses due to brucellosis. ©2019 PVJ. All rights reserved

Comparative Association of slc11a1 (Nramp1) Gene with Susceptibility and Resistance to Brucellosis in Various Cattle and Buffaloes Breeds of Pakistan