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Received: Revised: Accepted: Published online: August 31, 2018 November 26, 2018 December 19, 2018 January 24, 2019 Brucellosis is a zoonotic disease that led to economic losses to cattle industries worldwide including Indonesia. A rapid, precise and accurate diagnosis technique is required for early detection and for the control of brucellosis in animals and humans. The objective of this study was to molecularly identify and characterize B. abortus in the blood samples obtained from seronegative dairy cattle that have had reproductive disorders in Central Java, Indonesia, using PCR, phylogenetic and nucleotide sequence analyses of the IS711 regions. Results showed that in seronegatively dairy cattle with reproductive disorders, B. abortus was successfully detected from the whole blood and serum samples without separation of the buffycoat and without isolation of the organism by conventional PCR. The result also showed high homology level (up to 100%) and close phylogenetic relationships between nucleotide sequences of the IS711 gene fragment of B. abortus local isolates compare to the isolates that have been accessed in GenBank (CP 009099, CP 023242, CP 023242, CP 023308 and LT 671513). We concluded that PCR technique is useful for routine diagnosis of brucellosis in seronegative dairy cattle with reproductive disorders. ©2018 PVJ. All rights reserved

pakistan veterinary journal

Molecular Identification of Brucella abortus Collected from Whole Blood Samples of Seronegative Dairy Cattle with Reproductive Disorders in Central Java, Indonesia