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The problem of resistance is increasing for all types of bacterial isolates including Staphylococci. Methicillin resistant Staphylococcus aureus (MRSA) has emerged as an important hospital acquired and community acquired pathogen. Multi drug resistance among MRSA is a serious issue limiting the treatment options for the victims. MRSA strains that share the presence of staphylococcus cassette chromosome mec (SCC mec) in their genomes are frequently virulent and predominantly cause infections. Staphylococcal enterotoxins are a family of structurally related proteins that are produced by Staphylococcus aureus playing a major role in the pathogenicity. These microbial superantigens have profound effects on the immune system, which makes them useful tools for understanding its mechanism of action. The proposed study is mainly focused on collection of MRSA strains from clinical samples, isolation and Identification was done on the basis of microscopic examination and cultural characteristics, biochemical tests. The cultures were subjected for DNA extraction fallowed by AGE. All the samples were amplified targeting the gene Mec A and sent for sequencing. All the sequences were further analyzed and confirmed. The production and purification of Enterotoxins by MRSA was done and crude protein was collected. This was further purified by downstream processing. The sample was subjected for centrifugation followed by salt precipitation, Dialysis. The enterotoxins were produced form all the strains isolated and purified.

Production, Purification & Analysis of Enterotoxins from HA-MRSA Isolated from Clinical Samples