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A quasi-colloidal gold staining assay was carried out by preparing colloidal gold labeled 2C10 by preparing monoclonal antibody 2C10 with anti-tilapia immunoglobulin IgM. by Preparation of monoclonal antibody 2C10 against tilapia immunoglobulin IgM and marking the 2C10 with colloidal gold for detecting antibody of Streptococcus agalactiae (S. agalactiae) infection in tilapia. After the experimental test, the rapid immunogold colloidal gold filter detection method, the sensitivity and conventional ELISA equivalent to 1.2 × 10-6 g/mL protein; specificity is good, not with the common bacteria Aeromonas hydrophila, Pseudomonas fluorescens reaction; 4°C to preserve the shelf life of more than 3 months. Immune colloidal gold infiltration method Immunity colloidal gold infiltration method has the advantages of rapid and easy operation. The method takes only 5 minutes to detect the sample, only 3μL is needed to detect the sample. The detection of the method does not require special equipment and equipment, And can be detected on site. The establishment of the law provides a new method for the monitoring of tilapia-free Streptococcus disease, which is of great practical significance in controlling the risk of Streptococcus agalactiae. DOI: 10.29011/BBOA-107. 100007

Development an Immune Colloidal Gold Filtration Assay for Detecting Antibody of S. agalactiae in Tilapia