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The aim of the study was to compare contents of individual protein fractions determined by electrophoresis in blood serum of healthy periparturient goats. Eight clinically healthy white shorthaired goats were examined. Blood samples of these goats were taken from v. jugularis three weeks and two weeks before the anticipated parturition, on the parturition day, 7 days after the parturition, and 28 days after the parturition. Individual protein fractions, albumins, alpha 1-, alpha 2-, beta 1-, beta 2- and gamma-globulins were identified by electrophoretic analysis of blood serum. Percentage shares of individual protein fractions were converted from total protein values to g.l(-1) values. The results indicated that individual serum protein fractions in periparturient goats showed significant dynamics of change. There were no significant differences between samples taken before the parturition and on the parturition day (except for the increase of beta 2 from 2.5 +/- 0.3 g.l(-1) observed 3 weeks before the parturition to 3.2 + 0.4 g.l(-1) measured 2 weeks before the parturition, and the decrease of gamma G levels from 9.5 +/- 2.6 g.l(-1) observed three weeks before the parturition to 8.1 +/- 1.7 g.l(-1) found on the parturition day), yet most indicators measured after the parturition were significantly higher (p < 0.05) than on the parturition day (values in g.l(-1) observed on the parturition day versus values measured 28 days after the parturition: total protein 60.6 +/- 4.3 vs. 71.3 +/- 2.4, albumins 37.4 +/- 2.9 vs. 42.7 +/- 1.0, alpha 2 3.4 +/- 0.5 vs. 4.1 +/- 0.8, beta 1 5.6 +/- 0.5 vs. 6.3 +/- 0.9, gamma G 8.1 +/- 1.7 vs. 12.3 +/- 1.9). The results bring new knowledge on the values of individual fractions of serum proteins in healthy goats, which is necessary for diagnostic interpretation of pathological findings in animals and also for experimental studies.

Levels of protein fractions in blood serum of periparturient goats