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The aim of this study was to verify the application of polymerase chain reaction method focused on the detection of genus-specific tuf-gene for the rapid identification of enterococci. The reaction was as duplex-PCR, when, apart from the detection of genus-specific tuf-gene (amplification product of 112 bp), the internal control for 16S rRNA gene (amplification product of 241 bp) was also inserted. Detection of tuf-gene was positive in Enterococcus spp. isolates from the collections of microorganisms (8 species) and in 283 field isolates from foods and food raw materials, drinking water and various kind of waste water, previously identified by phenotypic methods as enterococci of different species. PCR product of 112 bp was missing in 16 non-enterococci strains and in 14 field isolates thereafter identified as lactococci. The above PCR method can be recommended for the rapid identification of enterococci in the routine use.

Genus-Specific Identification of Enterococci by PCR Method