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Lobová D., A. âíÏek: Susceptibility of Brachyspira hyodysenteriae Isolates to Doxycycline using Agar Dilution and Epsilometer Test. Acta Vet. Brno 2004, 73: 329-333. The agar dilution method and the Epsilometer test were used to evaluate antibacterial susceptibility of 49 Brachyspira hyodysenteriae field isolates to doxycycline. A comparison of results obtained by the two methods revealed agreement for 84% of B. hyodysenteriae isolates; with 16% of isolates the Minimal Inhibitory Concentration values differing by two and more dilutions. Out of the total of B. hyodysenteriaeisolates tested, 36 (73%) were classified as sensitive, 13 (27%) as intermediary, and none of the strains were found doxycycline-resistant. The demonstrated susceptibility of B. hyodysenteriae isolates to doxycycline suggests the possibility of using it for combination therapy with pleuromutilins of swine dysentery. The Epsilometer test has proved a practical alternative to dilution methods of B. hyodysenteriaesusceptibility testing. Etest, swine dysentery, Minimum Inhibitory Concentration, antibiotics