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Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P<0.05 and P<0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P<0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P<0.01 and P<0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P<0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production. Key words: cattle; cortisol; hair; blood; milk KONCENTRACIJA KORTIZOLA V DLAKI, KRVI IN MLEKU KRAV CRNO-BELE PASME IN PASME BUSA Ugotavljali smo koncentracijo kortizola v dlaki, krvi in mleku pri govedu dveh pasem, ki se razlikujeta po mlecnosti in pogojih reje. Raziskavo smo izvedli na kravah in telicah crno-bele pasme in pasme busa. Koncentracijo kortizola smo ugotavljali z imunoencimskimi metodami. Akumulacijo kortizola smo dolocili v proksimalnem (bliže koži) in distalnem (dlje od kože) delu dlake. Obenem smo ugotavljali vpliv barve in pranja dlake na koncentracijo kortizola. Tako v proksimalnem kot v distalnem delu dlake je bila koncentracija kortizola pri kravah crno-bele pasme v primerjavi s kravami pasme busa statisticno znacilno visja (P<0,01 in P<0,05). Pri primerjavi koncentracije kortizola med crno in belo dlako crno-belih krav nismo ugotovili statisticno znacilnih razlik. V dlaki, ki smo jo pred izvedbo dolocanja koncentracije kortizola oprali z izopropanolom, smo v primerjavi z neoprano dlako ugotovili statisticno znacilno nižjo vrednost kortizola (P<0,01). Rezultati torej kažejo, da je koncentracija kortizola v dlaki odvisna od nacina priprave vzorca (pranje dlake), barva dlake pri crnobeli pasmi pa ne vpliva na koncentracijo kortizola.. V krvnem serumu krav in telic crno-bele pasme je bila koncentracija kortizola statisticno znacilno visja (P<0,01 in P<0,05) kot pri kravah in telicah pasme busa. Tudi v mleku krav crno-bele pasme je bila koncentracija kortizola statisticno znacilno visja (P<0,05) kot pri kravah pasme busa. Predvidevamo, da je visji nivo kortizola pri crno-beli pasmi rezultat intenzivne reje in fizioloske prilagoditve na visoko mlecnost. Kljucne besede: govedo; kortizol; dlaka; kri; mleko

CORTISOL CONCENTRATIONS IN HAIR, BLOOD AND MILK OF HOLSTEIN AND BUSHA CATTLE