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Different concentrations of aqueous and ethanolic extracts of Ocimum tenuiflorum L. leaves were evaluated for their sucrase inhibition and non-enzymatic glycation inhibitory effects. The results revealed that aqueous extracts, particularly the one from oven-dried leaves had significant inhibition potential for all three assays. The IC50 values for sucrase inhibition assay, BSA-glucose assay, and BSA-fructose assay were, 370.48±2.14 μg/ml, 188.19±2.52 μg/ml, and 166.16±1.99 μg/ml, respectively and the highest inhibition found in1250 μg/ml were, 91.88±0.16%, 118.46±0.27%, and 104.15±0.21%, respectively. Other than that, the aqueous extract of fresh leaves and 50% ethanol extract of oven dried leaves also showed good inhibition activity. Though, the results presented the highest inhibition activity for BSA-glucose assay. The leaves also attained 50% inhibition with the lowest concentration for BSA-fructose assay. The overall result of O. tenuiflorum leaves suggested that it can be used for treating diabetes mellitus and the complications associated with this.

Evaluation of in vitro sucrase Inhibitory and non-enzymatic glycation properties of Ocimum tenuiflorum L. leaves