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Background and Aims: The silver nanoparticles (Ag NPs) synthesized by green synthesis have antimicrobial properties and their potential to be used in the medical field is quite high. Puse, also called “black doctor”, is widely used as a treatment for the health problems of animals and in folk medicine in Turkish nomad culture. It has been reported that Ag NPs biosynthesized with Juglans regia leaf extracts have antibacterial activity. The aim and importance of this study is the preparation of a mixture of "puse" and Ag NPs for the first time and the investigation of its antibacterial activity. Methods: In this paper, Ag NPs were synthesized by using J. regia leaves and morphological characteristics of the NPs obtained were determined using TEM. The formation of NPs was identified using an UV-Vis spectrophotometer. The antibacterial activity was investigated by combining the obtained and characterized Ag NPs with the "puse". The antibacterial activity of the mixture was tested on Gram (+) bacteria and Gram (-) bacter. Results: The mixture that was prepared with puse and Ag NPs showed higher antibacterial activity than antibiotics. Conclusion: The positive results showed that this mixture has the potential to be used as a preparation especially in the treatment of animal wounds and the skin infections caused by bacteria.

Enhancing the antibacterial activity of the biosynthesized silver nanoparticles by “püse”