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There is increasing interest in the endoscopic study of tissue laser-induced autofluorescence in the detection of malignancy. Flavins, NADH, and collagen have been regarded as a major source of fluorescence in colonic tissue. However, the basis of the different autofluorescence between normal and cancerous tissues is unknown. Human colonic tissues were obtained from resected specimens. To study the existance of fluorescence, frozen sections of normal and cancerous tissues were observed by fluorescence microscopy under 400-440 nm excitation. The strong autofluorescence was seen from the submucosal layer corresponding with the collagen. Tissue fluorescence did not decrease in a reductant or acid solution. These results indicate that flavines and NADH do not affect tissue fluorescence. In cancerous tissue, decrease of autofluorescence arose from the collagen in the submucosal layer, which was substituted by cancer cells, was recognized 緒言 近年、癌の早期発見、早期診断の重要性が指 摘されるなか、様々な方法、工夫が報告 されて お り、蛍光内視鏡 もそのひ とつの有力な手段 と して注 目されてきている。蛍光内視鏡は正常部 と病変部の自家蛍光の差を利用 した内視鏡装置 であ り、現在まで、気管支や大腸に対す る癌診 断への応 用が報告 されている1、2)。 この蛍光 内視鏡 にお ける病 変部の 自家蛍光の差 と して現 在 、フ ラビン、NADH、 コラーゲ ンがその有 力 な蛍光物質 とされてい るが3、4、5)、 その原理 、 機序 はい まだ明らかに されていない。今回、我 々 は ヒ ト大腸組織 を用 いて蛍光内視鏡(Ex437、 Em520)で 見 られ る自家蛍 光の差 を明 らか に

Autofluorescent appearance of normal and cancerous tissues in human colon by fluorescence microscopy