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Objectives: This investigation aimed to study the effect of rosemary essential oil on the transdermal absorption of crosslinked hyaluronic acid in vitro. Material and methods: The most suitable concentration of crosslinked hyaluronic acid was chosen and enhancing effects of rosemary essential oil on the permeation of crosslinked hyaluronic acid were evaluated. Results: The optimization of carbazole sulfate method by the 96-well plate was used to establish the standard curve of hyaluronic acid successfully. Crosslinked hyaluronic acid containing 5% (v/v) rosemary essential oil showed better skin penetration than blank control group in transdermal experiments. The combination of 5% (v/v) rosemary essential oil and 2%(v/v) azone as a permeability aid can achieve more effect than 5%(v/v) rosemary essential oil as a permeability aid. Conclusion: This study showed the enhancing effect of rosemary essential oil on crosslinked hyaluronic acid percutaneous absorption.

international journal of frontiers in medicine

Exploring the potential of rosemary essential oil as an natural penetration enhancer for promoting transdermal absorption of crosslinked hyaluronic acid in-vitro