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Use of synthetic fertilizers bring environmental pollution, increase weed growth, reduce atmospheric oxygen levels, decrease the fertility of agricultural land, causing eutrophication and run-offs, also even damaging coral reefs. Recent biotechnology approaches can be utilized to create a new variety of self-fertilization plants that allows it to supply its own nitrogen making it possible to overcome nitrogen problems and avoid them. This review aims to understand how far genetic engineering approach can be used by the introduction of nif regulon from Klebsiella penumoniae. The nitrogenase-encoding genes which make up the nif regulon proved can be inserted into the mitochondrial genome as like Allen et al. (2017) did in their research. The western blotting result showed that the research succesfully integrated the nif regulon to the genome and expressed their proteins.

Prospek Introduksi Regulon nif dari Bakteri Klebsiella pneumoniae ke Dalam Genom Mitokondria Tanaman