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The toxicity and PAH of C.gariepinus (0.9±0.01g) to 1, 3, 6, 9, 12 and 0.0 ml/L of BLCO oil was determined. There was no mortality in the control but 10, 20, 50 and 60% occurred in crude oil after 96 h exposure. The logarithmic probit line and R: y = 1.372x + 3.724 , R2 = 0.997 and 96-h LC50: 9.6 ml/L represented toxicity value for crude oil. Total PAH in exposed fish gave a value of 99.68±4.81 ng/μL The lowest value of 0.061(0%) Nap to the highest value of 384.68 (84%) in DahA for crude oil was determined but mean ∑ 16PAH gave 6% compared to the 11% that of its mean∑ 8PAH. The least observed difference LOD of PAH among 8PAHs was 0.02ng/μL and mean TEQ and MEQ gave 254.67 and 44.84 both corresponding to 11%. BaP-TEQ in BLCO ranged from 0.05(0%) in Chry to 1923(84%) in DahA. while BaPMEQ ranged 0.09 (0%) Chry -119.25 (30%) DahA. The respective Benzo [a] Pyrene carcinogenic and mutagenic equivalents of Clarias gariepinus to BLCO was 2 and 9% respectively. In conclusion, the 96h median lethal concentration of BLCO to C.gariepinus fingerlings was 9.6 ml/L in which its mean ∑ 16PAH gave 6% compared to 11% that of mean 8PAH. Mean TEQ and MEQ in 8PAH both corresponded to 11% however BaP-TEQ and BaP-MEQ in BLCO ranged respectively from 0% in Chry to 84 and 30% in DahA. The respective Benzo [a] Pyrene carcinogenic and mutagenic equivalents of Clarias gariepinus to BLCO was 2 and 9% .

sdrp journal of aquaculture, fisheries and fish science

Benzo [a] Pyrene- carcinogenic and mutagenic equivalents of fingerlings of Clarias gariepinus (Burchell, 1822) exposed to water soluble fractions of Nigerian Bonny Light crude oil BLCO