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Aquaponics is innovative recirculation system where hydrobionts are cultivated together with the plants. The possibilities for control of pathogens in these systems are highly restricted. One possible strategy for inhibition of pathogenic microorganisms in aquaponics is the usage of seaweeds extracts. The study connected with the investigation of inhibition effect of seaweeds from Bulgarian Black sea cost on different pathogens is rare. The aim of current study was to test In vitro the inhibition effect of three seaweed species (Ulva rigida, Cladophora vagabunda, and Ceramium rubrum) distributed at Black sea in front of Bulgarian cost on different pathogens (bacteria аnd fungi) which are potentially harmful to hydrobionts, plants and consumer of aquaponics products. The ethanol and methanol extracts from investigated seaweed species were prepared. They were tested with agar well diffusion method against the following fish, food borne and plant pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Salmonella typhimurium, Candida albicans, Penicillium verrucosum var. verrucosum, Fusarium graminearum, Fusarium moniliforme and Aspergillus ochraceus. The current study showed that the following extracts from seaweeds distributed in front of Bulgarian Black sea cost possess high inhibition effect (size of inhibition zone higher than 10 mm) against potentially pathogenic microorganisms in aquaponics: ethanol extract of C. vagabunda against B. cereus and A. ochraceus, methanol extract of C. vagabunda against C.albicans, ethanol extract of C.rubrum against E.coli, B.cereus and C.albicans.

romanian biotechnological letters

In vitro test of inhibition effect of extracts from three seaweed species distributed at Black sea on different pathogens potentially dangerous for aquaponics