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In F1 hybrid seed production, the elimination of contaminated self and/or sib-crossed seeds from hybrid seeds is an important subject. We attempted to use RFLP analysis of nuclear DNA for checking the seed purity of the F1 hybrid cucumber. Between the two parental lines which are promising for an F1 cucumber cultivar, two RFLP clones were established. The frequency of RFLP clones in the genomic library was 1%. Two micrograms of DNA, which could be isolated from a 9-day-old seedling, were sufficient for RFLP detection with non-radioactive Southern hybridization. This system enabled a practical and a rapid check of the seed purity in an F1 hybrid cucumber.

An Approach for Rapid Checking of Seed Purity by RFLP Analysis of Nuclear DNA in F1 Hybrid of Cucumber (Cucumis sativus L.)