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Taro (Colocasia esculenta) callus was induced from shoot tip explants on a modified Nitsch medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzyladenine (BA) at 1 mg•liter-1 each. The effect of agar concentration (0, 0.2, 0.4, 0.6, 0.8%, W/V) in a modified Murashige and Skoog (MS) medium on the regeneration of shoots from taro callus was investigated. Shoot differentiation was observed only in 0.2, 0.4 and 0.6% agar concentrations. A high level of shoot differentiation at 64% was measured on 0.6% agar medium. However, root differentiation was observed regardless of agar concentrations.Shoot differentiation was observed on the medium supplemented with 1 mg•liter-1 BA as early as 10 weeks of culture.Addition of 0.1% (W/V) charcoal did not directly induce the regeneration of shoots from the callus, but lengthened the longetivity of the calli since no necrosis was observed after 20 weeks of culture.Proliferation of regenerated shoots was enhanced with the addition of naphthalene acetic acid (NAA) and BA at 1 mg•liter-1 each. The plantlets can be readily potted in a vermiculite medium for acclimatization before planting in the field.

Plantlet Regeneration from Taro (Colocasia esculenta Schott) Callus.