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Induction of embryogenic callus was achieved from the basal plate with a shoot apex of the bulb and the aerial bulblet, and from a receptacle tissue in garlic (Allium sativum L.). Embryogenic callus was obtained from the basal plate on AZ medium supplemented with 1 to 10μM 2, 4-D, 10μM p-CPA and 1μM kinetin. The transformation was promoted more so by 2, 4-D than by p-CPA as the supplementary auxin. Embryogenic callus was induced from a garlic receptacle on BDS medium supplemented with 1 to 10μM NAA. BA was found to be inhibitory to induction of embryogenic callus. Somatic embryogenesis by calli obtained on the above media was confirmed by histological examinations. Large number of pro- and globular-stage embryos, some having irregular shapes, were observed. Some regenerated plants obtained from embryogenic calli on a medium supplemented with 1μM NAA and 5 to 10μM BA were successfully transferred to soil and grown to maturity.

Somatic Embryogenesis and Plant Regeneration in Basal Plate and Receptacle Derived-Callus Cultures of Garlic (Allium sativum L.).