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Investigations were carried out to clarify the effects of root temperature on nitrogen contents in potted or benched `Muscat of Alexandria' vines grafted on `Hybrid Franc' rootstock. Root temperature was controlled to 13°C and 2TC from December and from February, under forcing conditions. Seasonal changes in nitrogen contents in unheated vines were also determined for comparison. 1. In unheated vines, the seasonal change in insoluble nitrogen content of all oranges such as rootlets, lateral roots, trunk, and cane, was small, though the contents of soluble and amino nitrogens in roots increased gradually toward the bursting stage, and also increased at that stage in canes. In heated vines, the insoluble nitrogen content in roots decreased markedly after the start of the treatments in all plots, especially at 27°C, but only a slight difference in the soluble nitrogen content was observed. In forcing from both December and February, amino nitrogen content in rootlets was markedly lower at 13°C than at 27°C. At 13°C from December, amide content in rootlets was notably low. Little difference in the contents of insoluble and amino nitrogens in canes was found between the two temperatures for forcing from both December and February , whereas soluble nitrogen content in canes began to decrease before the bursting stage at both root temperatures from December, especially at 27°C, where budbreak was promoted. 2. The concentration of total nitrogen in bleeding sap was high at leafing stage of the heated ones. At 13°C from December, nitrogen concentration and the ratio of amino nitrogen to total nitrogen in sap were very low. 3. The contents of total, insoluble and amino nitrogen in shoots were greater in the forcing from February than from December, and at 27°C than at 13°C . There was little difference between the two temperatures in the soluble nitrogen content of shoots, regardless of the time of forcing. At 13°C from December, where growth of shoots and clusters was markedly inhibited, the amide content was notably low. 4. In vines forced from December, 15N content in both soluble and insoluble fractions in rootlets, canes, and shoots was markedly greater at 27°C than at 13°C. That is, 15N content in the insoluble fraction in shoots treated at 27°C was about twice as much as that at 13°C.

Effects of Root Temperature under Forcing Conditions on Nitrogen Contents in ^|^lsquo;Muscat of Alexandria^|^rsquo; Vines