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In order to obtain Cattleya protocorms that are uniform in both physiological and morphological aspects, in vitro tissue culture method was employed. Shoot apexes were aseptically dissected out of the newly developed buds and subcultured on solid and in liquid media. Several combinations of growth regulators and organic nutrient sources were added to the culture media to look for the composition of medium most effective for organogenesis and proliferation of protocorms. The results obtained are shown below.1) Four selected combinations of growth regulators each proved effective when they were added to the basal medium at proper concentrations. If growth regulators were combined with 2, 4-D, however, effective concentration of growth factors fell within a narrow range.2) The composition of culture media most effective for the proliferation of protocorms was fairly different from that most suitable for shoot formation of propagules.3) Supplementation of BA combined with NAA was most effective for protocorm proliferation. That is, the media including BA at 5.0mg/l and NAA at 1.0mg/l caused maximum proliferation of proctocorms, though wider ranges of media concentrations were also capable of exerting stimulative effects. Besides, addition of BA alone at 0.1mg/l to the culture medium showed noticeable effects on the proliferation of protocorms.4) Shoot formation of propagules was most stimulated by supplementation of BA at 0.1mg/l combined with 2, 4-D at 0.1mg/l. In the combination of 6-benzyladenine with NAA, however, the culture media were effective for shoot formations within a relataively wide range of concentrations, e.g., BA at 0.1 to 1.0mg/l and NAA at 0.5 to 1.0mg/l.5) Supplementation of 2, 4-D alone at concentrations over 0.5mg/l killed the protocorms completely, though combination of kinetin or 6-benzyladenine with 2, 4-D seemed to alleviate the toxic effect of 2, 4-D.6) Organic supplements such as banana juice and raw coconut milk produced a prominent proliferation of protocorms. Effects of organic supplements on shoot formation, however, were less than those of growth regulators. Addition of yeast extract to the culture media rather inhibited the shoot formation of propagules.

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Effects of Combinations of Growth Regulators, and of Organic Supplements on the Proliferation and Organogenesis of Cattleya Protocorm Like Bodies Cultured in vitro