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Sorghum serves as staple food for over 100 million people in Sub-Saharan African countries. It is the most important nutritional security crop and ranks third among major cereal crops in terms of area and production next to teff and maize in Ethiopia. However, Sorghum is susceptible to contamination by molds that produces aflatoxin that causes hepatotoxic and carcinogenic effects on humans and animals. This study was conducted to assess Aspergillus species and aflatoxin level in Sorghum (Sorghum bicolor L.) stored under different storage system for different storage period. Thirty samples were analyzed for aflatoxin contamination using high performance liquid chromatography equipped with fluorescent detector and Aspergillus species were isolated and identified using phenotypic features in a potato dextrose agar culture media. About 56.7%, 16.7%, and 23.3% of the Sorghum samples were found to be contaminated with Aspergillus flavus, Aspergillus niger and Aspergillus parasiticus, respectively. The level of total aflatoxin, aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2 were in the range of 11.44 to 344.26μg/kg, 3.95 to 153.72μg/kg, 1.17 to 91.82μg/ kg, 9.87 to 139.64μg/kg, and 3.22 to 52.02μg/kg, respectively. The concentration of aflatoxin in all Sorghum samples surpassed the maximum level set by the European commission and therefore, deserves attention to control them across the Sorghum value-chain.

hsoa journal of food science and nutrition

Study on Aspergillus Species and Aflatoxin Levels in Sorghum (Sorghum bicolor L.) Stored for Different Period and Storage System in Kewet Districts, Northern Shewa, Ethiopia