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Patterns of VIII R: AG in the plasma, cryoprecipitate and cryosupernatant fractions from normal subject and von Willebrand's disease variants were investigated by SDS 1. 5 % polyacrylamide gel electrophoresis-crossed immunoelectrophoresis (SDS PAGE-CIE). Neutralization of SDS after PAGE was performed by inserting an intermediate agarose layer imprignated with Triton X-100. Molecular weight of VIII R: AG in normal plasma ranged from 8 x 105 to 8 x 106 daltons. Normal cryoprecipitate showed several precipitin peaks with a molecular weight range of 8 x 105 1 x 106 daltons. Normal cryosupenatant had two low peaks with 8 x 105 2 x 106 daltons. VIII R: AG in the plasma and cryoprecipitate fractions from von Willebrand's disease variants, showing fast anodal mobility in the conventional CIE, gave 23 peaks with low molecular weights of 8 x 105 3 x 106 daltons. Plasma VIII R : AG in these patients were suggested to stay in low levels of polymerization/ aggregation of subunit and not to show VIII R: WF similar to VIII R : AG in normal cryosupernatant.

Molecular size of factor VIII-related antigen in normal plasma and in plasma of von Willebrand's disease variants