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Wistar male rats were repeatedly exposed to methanol and toluene vapors individually and simultaneously by inhalation 6 hours a day, five days a week for 4 weeks. Blood was obtained from the tail of the rats up to 23 hours after the end of 4-week exposure and the methanol and toluene concentrations were measured. Major metabolites of methanol and toluene, that is, formic acid and hippuric acid in urine were measured up to 6 days after the end of 4-week exposure. The biological half time of toluene in blood in the simultaneous exposure group was shorter than that in the toluene exposure group. This tendency was almost the same as that for one-day exposure, although the biological half time of solvents in the rat blood was prolonged. The half times of methanol were also longer than those for one-day exposure.

Effect of Repeated Exposure to Methanol and Toluene Vapor on the Metabolism of Rats.