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ABO blood group antigens are involved in various biological phenomena, including immune responses and infections. We achieved efficient and scalable synthesis of A, B, and H antigens. Using chemical conjugation, synthesized B antigen was displayed on the surface of living cells and its function as an antigen was confirmed by the IgM antibody recognition. Our results indicate that the multivalent interactions induced by cell surface glycan clustering are crucial in this system. The prepared cells displaying the glycan antigen are expected to be a model cell for investigating ABO antigen function.

Synthesis of ABO blood group antigens and functional glycan display on the cell surface