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The small field experiment with broccoli (variety ‚Tiburon F1‘) was established in The Botanical Garden of Slovak University of Agriculture in Nitra. In this experiment, we investigated the effect of four variants of nutrition on the broccoli yield, content of sulforaphane and vitamin C in broccoli florets. The first variant of nutrition was a control one - without application of fertilizers. The second one was fertilized at nitrogen level 200 kg.ha-1. The third one had level of nutriments N : S = 200 : 50 kg.ha-1 and fourth one N : S = 200 : 60 kg.ha-1. In each variant of nutrition, the yield of broccoli was significantly increased in comparison with the control variant. The highest yield of broccoli florets was reached in variant 4 (27.87 t.ha-1). The highest amount of sulforaphane was also determined in variant 4 - 50.93 mg.kg-1 of fresh matter. However, this value did not represent significant increase of sulforaphane content compared to the control variant. The highest content of vitamin C was determined in variant 2 (585.78 mg.kg-1 of fresh matter). In this variant, we achieved significant increase of vitamin C content in comparison with the control variant. Thus, by application of nitrogen and sulphur nutrition, we reached significant increase of broccoli yield compared to the control variant. At the same time, the applied nutrition assisted to enhanced accumulation of sulforaphane and vitamin C in broccoli florets Maloparcelkový poľný pokus s brokolicou (odroda Tiburon F1) bol založený v roku 2008 v areáli Demonštračnej záhrady SPU v Nitre. V pokuse sme skúmali vplyv štyroch variantov výživy na úrodu, obsah sulforafanu a vitamínu C v ružiciach brokolice. Prvý variant (1) bol kontrolný - neboli na ňom aplikované priemyselné hnojivá. Variant 2 bol vyhnojený na úroveň N 200 kg.ha-1. Pri 3. a 4 variante boli živiny doplnené na úroveň N : S = 200 : 50 kg.ha-1, resp. 200 : 60 kg.ha-1. Na všetkých variantoch výživy sa úroda ružíc brokolice preukazne zvýšila v porovnaní s kontrolou. Najvyššia úroda ružíc brokolice bola dosiahnutá na variante 4 - 27,87 t.ha-1. Najväčšie množstvo sulforafanu bolo taktiež zistené vo variante 4 - 50,93 mg.kg-1 čerstvej hmoty. Táto hodnota však nepredstavovala preukazné zvýšenie obsahu sulforafanu oproti kontrolnému variantu. Najväčší obsah vitamínu C bol dosiahnutý na variante 2, pričom sme zaznamenali preukazné zvýšenie obsahu vitamínu v porovnaní s kontrolou. A teda, aplikáciou dusíkatej a sírnatej výživy sme dosiahli preukazné zvýšenie úrody oproti kontrolnému variantu, ale zároveň aplikovaná výživa prispela k zvýšenej kumulácii sulforafanu a vitamínu C v ružiciach brokolice

acta horticulturae et regiotecturae

The Effect of Nitrogen and Sulphur Nutrition on the Yield and Content of Antioxidants in Broccoli / Vplyv Dusíkatej a Sírnatej Výživy na Úrodu a Obsah Antioxidantov v Brokolici