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Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes ( BCL2 ,  BAX ,  p53 ,  CASP9 ) during the seven days of primary  in vitro  culture of GCs isolated from patients undergoing  in vitro  fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points. Running title : Apoptosis-related genes expression in granulosa cells  in vitro

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells