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The greatest rooting of Gypsophila paniculata occurred in various propagation media with a pH near, or slightly above, neutrality and poorest rooting in acidic media. Addition of ground limestone to acidic media significantly (P ≥ 5%) improved rooting. Basal dipping of cuttings in H2SO4 (sulfuric acid), NaOH (sodium hydroxide), and KOH (potassium hydroxide) resulted in improved rooting over nontreated cuttings. Lignin specific phloroglucinol staining of freehand sections revealed the presence of a band of sclerenchyma tissue, external to the vascular region of the stem, that may impede the emergence of newly formed roots.

Effects of Media PH and Acid/Base Pretreatments on the Rooting of Gypsophila Paniculata Cuttings