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The low concentration of copper (Cu2+) can be effected on the central nervous system (CNS) and caused to multiple sclerosis (MS). Although many antibiotics can treat the bacterial infections but some of antibiotics decrease essential metal concentrations in human body and must be controlled by determining. In this study, in-vitro extraction of copper (Cu2+) with amoxicillin/clavulanic acid (AMOXC) has been studied due to interacting with metals. By procedure, Cu2+ ions were separated from blood samples by ultrasound assisted-dispersive centrifuge liquid-liquid micro extraction (USA-DC-LLME). The mixture of AMOXC (0.01 g), ionic liquid ([BMIM][PF6]) and acetone injected to 10 ml of serum blood sample at human pH=7.2. After extraction, the concentration of Cu2+ ions was determined by flame atomic absorption spectrometry (F-AAS). The LOD, enrichment factor (EF), linear range (LR) and working range (WR) were obtained 6 μg L-1, 9.92, 0.018-0.5 mg L-1 and 0.02-2.58 mg L-1, respectively (RSD<1.1%).

analytical methods of environmental chemistry journal

In-vitro extraction and separation of copper ions from human blood samples based on amoxicillin/clavulanic acid by ultrasound assisted-dispersive centrifuge liquid-liquid micro extraction