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The ultrasound assisted- dispersive solid phase microextraction method (USA-SPME) was used for in-vitro study on separation/extraction of calcium ions in human blood of chronic kidney disease (CKD). In this procedure, amine-functionalized bimodal mesoporous silica nanoparticle (NH2-UVM7) as a solid phase was used for in-vitro separation/extraction of calcium from blood/serum samples. Moreover, a mixture of NH2-UVM7 with ionic liquid and acetone (S/IL/Ac) was added to serum/blood sample containing of Ca (II) at pH of 7.3. After ultrasonic bath and centrifuging, NH2-UVM7/ IL settled down in bottom of tube, which was extracted Ca (II) ions by binding to amine group ([Ca]2+ →: NH2 ─ UVM7). The concentration of Ca (II) was determined by flame atomic absorption spectrometry (F-AAS, N2O, C2H2) after back extraction remained adsorbent in IL by 0.5 mL of HNO3 (0.5 M).

analytical methods of environmental chemistry journal

Dispersive solid phase microextraction based on aminefunctionalized bimodal mesoporous silica nanoparticles for separation and determination of calcium ions in chronic kidney disease