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A convenient method for the evaluation of potential antiviral agents against non-segmented negative single strand RNA viruses using infectious hematopoietic necrosis virus (IHNV) in chinook salmon embryo (CHSE-214) cells was performed on microtiter plates with 96 wells. In this system, cytopathic effect (CPE) induced by IHNV infection was detected as clear spots (CPE spot) in CHSE-214 cell monolayers three days after infection. In none of the cases tested, did the standard error for the number of CPE spots per well exceed 10%. This newly developed method was successfully applied to the quantitative evaluation of several antiviral agents against IHNV on microtiter plates (CPE spot reduction method), with a reproducibility equal to the plaque reduction method.

nihon suisan gakkaishi/nippon suisan gakkaishi

A new method for the evaluation of antiviral agents agains infectious hematopoietic necrosis virus (IHNV) on microtiter plates: CPE spot reduction method.