English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The effects of amygdalin as an herbal substance on prevention of cancer cells   proliferation it has been shown. Also, survivin gene is one of the important   genes on inhibition of apoptosis and controlling of the cell cycle in cancer   cell lines. For this purpose, the present study was undertaken for the first   time to evaluate the effects of amygdalin on survivin gene expression in   MCF-7 and HDF. The MCF-7 (Breast cancer cells) and normal HDF were treated by   different doses of amygdalin (0.5 to 10 mg/ml). Then, the MTT assay was done   on each cell groups on 24, 48, and 72 h after treatment. In each period time   the cells were detached and used for RNA extraction and cDNA synthesis.   Finally, the survivin gene expression in each cell groups was evaluated by   quantitative real-time PCR (q-RT-PCR) analysis. The MTT assay was showed that   5 mg/ml of amygdalin concentration at 48 hours after treatment it was   suitable for evaluation of this compound on MCF-7 and HDF cell lines. The   survey results of q-RT-PCR were showed in both amygdalin-treated MCF-7   (MCF-7+AMG) compare to the treated HDF (HDF-AMG as an control group) the   expression level of survivin gene were decreased but this reduction only in   MCF-7+AMG group was statistically significant (p &lt;0.05). Our findings   indicated that amygdalin in particular; decrease the survivin mRNA in MCF-7   breast cancer cells compare to normal healthy cells and leads to apoptosis   and the death of cancer cells. It is suggested that in future study it must   be better the effects of amygdalin supplemented with surfactant investigate   against other cancer cells and evaluate the more molecular markers to specify   the anti-cancer activity potential of this herbal compound.

Evaluation of the effect of amygdalin on survivin gene expression in MCF-7 breast cancer cell line